Imaging Fourier transform endospectroscopy for in vivo and in situ multispectral imaging

We report the design and implementation of a multispectral imaging Fourier transform endospectroscopy (IFTES) system. The IFTES system employs a flexible fiber bundle catheter coupled to a home-built imaging Fourier transform spectroscope. The instrument enables the performance of non- or minimally invasive subsurface imaging and multispectral imaging at the cellular level in vivo and in situ. A maximum spectral resolution of 0.2 nm at 632.8 nm and a lateral resolution of 4.4 μm were proved. Preliminary results of a standard resolution target, ex-vivo small animal tissue, single wavelength laser, fluorescence solution, in-vivo mouse skin, microspheres mixture, and in-vivo transgenic mouse brain were given to demonstrate the potential of the technique.