Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

633
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
633
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

775
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
775
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.4K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Bridge of Disconnect: An Angiographic and Cadaveric Study of Myocardial Bridges and an Overview of Its Embryology.

Cureus·2025
Same author

Wild house mice have a more dynamic and aerotolerant gut microbiota than laboratory mice.

BMC microbiology·2025
Same author

Occurrence, molecular characterization and tolerance of Cronobacter spp. isolated from fermented corn products in China.

Food microbiology·2025
Same author

Apical periodontitis microbiome association with salivary and serum inflammatory burden.

International endodontic journal·2025
Same author

Oral dysbiosis initiates periodontal disease in experimental kidney disease.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2024
Same author

Multiplex Determination of K-Antigen and Colanic Acid Capsule Variants of <i>Cronobacter sakazakii</i>.

Genes·2024

Related Experiment Video

Updated: Mar 2, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K

Insights into the Emergent Bacterial Pathogen Cronobacter spp., Generated by Multilocus Sequence Typing and Analysis.

Susan Joseph1, Stephen J Forsythe

  • 1Pathogen Research Group, School of Science and Technology, Nottingham Trent University Nottingham, UK.

Frontiers in Microbiology
|November 29, 2012
PubMed
Summary

Multilocus sequence typing (MLST) advances Cronobacter spp. identification, aiding in the recognition of new species and understanding disease links. This DNA-based method improves detection of this pathogen, crucial for protecting infant health.

Keywords:
CronobacterMLSAMLSTST4sequence typing

More Related Videos

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

11.4K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

9.1K

Related Experiment Videos

Last Updated: Mar 2, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

11.4K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

9.1K

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Cronobacter spp. (formerly Enterobacter sakazakii) is a significant bacterial pathogen, particularly dangerous to neonates and infants through contaminated infant formula.
  • Accurate detection and identification methods are crucial due to the pathogen's importance and regulatory oversight.
  • Understanding Cronobacter diversity is essential for developing effective control strategies.

Purpose of the Study:

  • To evaluate the utility of multilocus sequence typing (MLST) and multilocus sequence analysis (MLSA) for characterizing Cronobacter spp.
  • To leverage MLST for improved understanding of Cronobacter diversity, species delineation, and pathogen-host associations.
  • To highlight the advantages of DNA-based typing over traditional methods for emerging bacterial pathogens.

Main Methods:

  • Utilized multilocus sequence typing (MLST) analyzing seven housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB, ppsA) with a total length of 3036 base pairs.
  • Employed multilocus sequence analysis (MLSA) to interpret the sequence data and assess genetic relationships within the Cronobacter genus.
  • Leveraged the curated and publicly accessible MLST database (http://www.pubMLST.org/cronobacter) for data analysis and comparison.

Main Results:

  • MLST and MLSA facilitated the description of Cronobacter diversity and the formal recognition of new species, including C. universalis and C. condimenti.
  • The study revealed a high degree of clonality among Cronobacter strains.
  • A significant association was identified between clonal complex 4 and neonatal meningitis cases.

Conclusions:

  • MLST provides a reliable and objective DNA-based approach for bacterial typing, surpassing subjective phenotyping methods.
  • MLST has been instrumental in advancing the understanding of the Cronobacter genus and its pathogenic potential.
  • The application of MLST contributes to improved control measures for protecting neonatal health against Cronobacter infections.