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Updated: May 16, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
[Effects of altitude on soil microbial community in Quercus liaotungensis forest]
Di Zhang1, Yu-Xin Zhang, Lai-Ye Qu
1State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. johnzd_1114@163.com
Abstract:
Taking the Quercus liaotungensis forest soil in Dongling Mountain of Beijing as the object, and by using chloroform fumigation-extraction and phospholipid fatty acid (PLFA) analysis methods, this paper studied the variation characteristics of soil microbial community along an altitudinal gradient in the tree growth season. With increasing altitude, the soil microbial biomass carbon and nitrogen and the quantities of various soil microbial groups in the forest had definite differences but not significant. The ratio of soil bacteria to fungi increased, but the ratio of G(+)- to G- bacteria decreased. The soil microbial biomass carbon and nitrogen and the quantities of soil bacteria, fungi, and G(+)- and G- bacteria had significant positive correlations with the contents of soil moisture, organic carbon, and total nitrogen, and the quantity of soil fungi was positively correlated with soil carbon/nitrogen ratio. The variations of the soil microbial community structure (bacteria/fungi and G(+)-/G- bacteria) were mainly affected by soil temperature and moisture content, which meant that the soil microbial community structure was sensitive to the environmental conditions. Along with the global warming, the proportions of soil fungi and G+ bacteria in the Q. liaotungensis forests in warm temperate zone would have an increase.
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