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AID in aging and autoimmune diseases.
Daniela Frasca1, Gianluca Andrisani, Alain Diaz
1Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 016960, USA.
Autoimmunity
|November 30, 2012
Summary
Inflammatory Bowel Disease (IBD) patients on combined anti-TNF-α and immunosuppressive therapy show significantly reduced B cell responses to influenza vaccines. This impairment is comparable to that seen in elderly individuals, highlighting potential vulnerabilities in their immune response.
Area of Science:
- Immunology
- Vaccinology
- Gastroenterology
Background:
- B cell responses are crucial for vaccine efficacy.
- Inflammatory Bowel Disease (IBD) treatments can impact immune function.
- The pandemic (p)2009 influenza vaccine response varies with age and treatment status.
Purpose of the Study:
- To assess B cell quality in IBD patients and healthy individuals post-influenza vaccination.
- To compare in vivo and in vitro B cell responses.
- To investigate the effect of anti-TNF-α and immunosuppressive (IS) drugs on B cell function.
Main Methods:
- Hemagglutination Inhibition (HAI) assay for in vivo antibody response.
- Activation-Induced Cytidine Deaminase (AID) measurement in vaccine-stimulated peripheral blood mononuclear cells (PBMCs) for in vitro response.
- Polyclonal stimulation with CpG as a potential biomarker.
Main Results:
- B cell responses were impaired in IBD patients on anti-TNF-α monotherapy.
- Responses were significantly more decreased in IBD patients on combined anti-TNF-α and IS therapy, similar to elderly individuals.
- In vitro AID activation correlated with serum HAI response, suggesting its utility as a biomarker.
Conclusions:
- IBD patients on combined anti-TNF-α and IS therapy exhibit markedly impaired B cell responses to influenza vaccine.
- B cell defects, indicated by AID activity, contribute to reduced humoral immunity in these patients.
- The findings suggest that B cell function assessment could inform vaccination strategies for IBD patients.
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