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DNA Microarrays02:34

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Carbohydrate Digestion00:57

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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Carbohydrate microarrays.

Sungjin Park1, Jeffrey C Gildersleeve, Ola Blixt

  • 1National Creative Research Initiative Center for Biofunctional Molecules, Department of Chemistry, Yonsei University, Seoul 120-749, Korea.

Chemical Society Reviews
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

Carbohydrate microarrays are essential tools for high-throughput analysis of glycan interactions. This review details their construction, detection methods, and diverse applications in biological and biomedical research.

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Area of Science:

  • Glycobiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Carbohydrate microarrays have emerged as key technologies for high-throughput analysis of carbohydrate-mediated events.
  • Various methods exist for immobilizing glycans onto solid surfaces in a microarray format.

Purpose of the Study:

  • To review the construction of carbohydrate microarrays.
  • To discuss detection methods for carbohydrate microarrays.
  • To highlight the applications of carbohydrate microarrays in biological and biomedical research.

Main Methods:

  • Review of existing literature on carbohydrate microarray construction.
  • Analysis of different detection strategies employed in carbohydrate microarrays.
  • Compilation of diverse applications in biological and biomedical fields.

Main Results:

  • Carbohydrate microarrays enable rapid analysis of lectin and antibody binding properties.
  • They facilitate quantitative measurements of glycan-protein interactions.
  • Applications include cell/pathogen detection, disease diagnostics, and enzyme specificity assessment.

Conclusions:

  • Carbohydrate microarrays are versatile platforms for studying complex biological recognition events.
  • Their applications span diagnostics, drug discovery, and fundamental biological research.
  • Continued development promises further advancements in understanding glycan roles.