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Highly polymorphic minisatellite DNA probes. Further evaluation for individual identification and paternity testing
J C Smith1, C R Newton, A Alves
1ICI Diagnostics, Northwich, Cheshire, United Kingdom.
Journal - Forensic Science Society
|January 1, 1990
Summary
This study validates DNA fingerprinting protocols using minisatellite probes, confirming reliable individual identification and Mendelian inheritance patterns in human DNA. Findings support the consistency and reproducibility of this genetic analysis technique.
Area of Science:
- Forensic Genetics
- Molecular Biology
- Human Genetics
Background:
- Minisatellite DNA probes enable highly polymorphic DNA fingerprinting.
- Established protocols are crucial for routine genetic analysis and identification.
Purpose of the Study:
- To validate and refine DNA fingerprinting protocols for routine use.
- To assess band sharing and inheritance patterns in human DNA.
- To provide further evidence for Mendelian inheritance of minisatellite loci.
Main Methods:
- Utilized minisatellite DNA probes (33.15 and 33.6) for DNA fingerprinting.
- Analyzed DNA fingerprints from 50 unrelated individuals to determine band sharing.
- Examined over 100 families to study the occurrence of mutant bands in offspring.
Main Results:
- Developed reliable and reproducible DNA fingerprinting protocols.
- Quantified band sharing among unrelated individuals and scorable bands per individual.
- Observed mutant bands in offspring, supporting Mendelian inheritance.
- Found no significant allelism or linkage between variable DNA fragments.
Conclusions:
- The developed protocols are suitable for routine DNA fingerprinting.
- Results reinforce the understanding of minisatellite DNA variability and inheritance.
- Supports the application of DNA fingerprinting in various genetic studies and forensic applications.