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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
Genome Copying Errors02:46

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...

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Reusable Single Cell for Iterative Epigenomic Analyses
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Clone DB: an integrated NCBI resource for clone-associated data.

Valerie A Schneider1, Hsiu-Chuan Chen, Cliff Clausen

  • 1National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA. schneiva@ncbi.nlm.nih.gov

Nucleic Acids Research
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

The National Center for Biotechnology Information (NCBI) Clone DB is a new resource for accessing genomic and cell-based clones. This database aids researchers in genome sequencing and variation analysis, offering detailed information and search tools.

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Area of Science:

  • Biotechnology
  • Bioinformatics
  • Genomics

Background:

  • The NCBI Clone Registry was a previous resource for accessing research clones.
  • Clones are essential reagents for various biological research applications, including genome sequencing and variation analysis.

Purpose of the Study:

  • To introduce the NCBI Clone DB, an integrated resource for accessing and utilizing research clones.
  • To replace and expand upon the former NCBI Clone Registry.

Main Methods:

  • The Clone DB integrates information on genomic and cell-based libraries and clones from over 100 eukaryotic taxa.
  • Records include details on library construction, associated sequences, map positions, and distribution.
  • The database is searchable via the NCBI Entrez system using fields like organism, clone name, gene name, and sequence identifier.
  • The NCBI Clone Finder tool allows searching for clones based on sequence coordinates or genomic features.

Main Results:

  • Clone DB provides comprehensive data on over 100 eukaryotic taxa.
  • Genomic clones are mapped to reference assemblies, with map positions available in records.
  • Searchable data facilitates the identification and utilization of specific research clones.

Conclusions:

  • The NCBI Clone DB serves as a centralized, comprehensive resource for researchers.
  • It provides essential tools for accessing and leveraging valuable research clones for diverse applications.
  • The database enhances accessibility and usability of clones for genome sequencing and variation analysis.