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Shear Assay Protocol for the Determination of Single-Cell Material Properties
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Published on: May 19, 2023

Do the microshear test variables affect the bond strength values?

Andrea M Andrade1, Eugenio Garcia, Sandra Kiss Moura

  • 1Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, Cidade Universitária, 05508-000 São Paulo, SP, Brazil.

International Journal of Dentistry
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

Specimen preparation impacts micro-shear bond strength (μSBS) testing. Removing the polyethylene tube before testing composite rods significantly affects results, influencing bond strength measurements in dental adhesives.

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Area of Science:

  • Dental Materials Science
  • Biomaterials Engineering
  • Adhesive Dentistry

Background:

  • Standardized protocols are crucial for reliable micro-shear bond strength (μSBS) testing.
  • The influence of polyethylene tube usage during specimen preparation on μSBS outcomes remains unclear.

Purpose of the Study:

  • To investigate the effect of polyethylene tube removal timing on μSBS results.
  • To determine if variations in specimen preparation influence the bond strength of dental adhesives to dentin.

Main Methods:

  • Human dentin disks were divided into six groups, varying polyethylene tube use and adhesive systems.
  • Composite rods were prepared with or without polyethylene tubes, tested after 24h water storage.
  • Micro-shear bond strength (μSBS) was measured, and data analyzed using two-way ANOVA and Tukey's tests.

Main Results:

  • No significant difference in μSBS was found between removing the tube after 24h (Group 1) and keeping it during testing (Group 2).
  • Group 1 exhibited a higher incidence of pretest failures compared to Group 2.
  • Composite rods placed without polyethylene tubes (Group 3) showed significantly higher μSBS values regardless of the adhesive system used.

Conclusions:

  • The removal of the polyethylene tube prior to placing the composite rod on dentin significantly impacts μSBS values.
  • Adhesive system choice did not influence the effect of tube removal on bond strength.
  • Standardized specimen preparation protocols are essential for accurate μSBS evaluation in dental research.