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Published on: June 26, 2014
Prevalence of hepatitis C virus genotypes in mashhad, northeast iran
H Vossughinia1, LA Goshayeshi, H Rafatpanah Bayegi
1Dept. of Gastroenterology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Insights
The most common Hepatitis C virus (HCV) genotype in Mashhad, Iran, was genotype 3a, followed closely by genotype 1a. This study clarifies HCV genotype distribution in this region.
Area of Science:
- Hepatology
- Virology
- Infectious Diseases
Background:
- Hepatitis C poses a significant global health challenge.
- Hepatitis C virus (HCV) genotype distribution in Mashhad, Iran, was previously unknown.
- Understanding regional genotype prevalence is crucial for effective treatment strategies.
Purpose of the Study:
- To determine the prevalence of HCV genotypes among seropositive patients in Mashhad.
- To investigate the relationship between HCV genotypes and patient demographic/virologic features.
Main Methods:
- Analysis of 382 clinical specimens from HCV-infected patients (2009-2010).
- HCV genotyping performed using Nested PCR amplification of the HCV core gene with specific primers.
Main Results:
- Hepatitis C virus (HCV) genotype 3a was the most prevalent (40%), followed by genotype 1a (39.2%).
- Other identified genotypes included 1b (10.9%), 5 (3.4%), and 2 (2.4%).
- Mixed genotypes (1a+1b, 1a+3a) and undetermined genotypes were found in a small percentage of patients.
Conclusions:
- Hepatitis C virus (HCV) genotype 3a is dominant in the Mashhad population.
- This research contributes to the understanding of predominant HCV genotypes across Iran.
Background:
Hepatitis C is a disease with significant global impact. The distribution of hepatitis C virus (HCV) genotypes in Mashhad (the Northeast and the biggest city after the capital of Iran) is unknown. The purpose of this study was to determine the prevalence of HCV genotypes among HCV seropositive patients, and to study the relationship between types, virologic and demographic features of patients in Mashhad.
Methods:
Three hundred and eighty-two clinical specimens obtained from HCV-infected patients referred to Ghaem Hospital in Mashhad during a period of 2009 to 2010 were selected. HCV genotype was determined by Nested PCR amplification of HCV core gene using genotype specific primers.
Results:
Totally, 299 patients were male (79.9%). The most common HCV genotype was genotype 3a, with 150 (40%) of subjects. Genotype 1a was the other frequent genotype, with 147(39.2%) subjects. Frequency of genotypes for 1b, 5 and 2 was 41(10.9%), 13(3.4%) and 9(2.4%), respectively. Mix genotype including 1a+1b in 4 (1.04%), 1a+3a in 3 (0.8%) was found in 7 patients. Four percent out of these samples had an undetermined genotype. Among the hemophilia patient, there were 13(48.1%) genotypes as 1a, 3(11.1%) 1b and 10(37%) 3a, respectively.
Conclusion:
The dominant HCV genotype among patients living in Mashhad was 3a. This study gives added evidence of the predominant HCV genotypes in Iran.
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