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Related Concept Videos

2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.

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Related Experiment Video

Updated: May 16, 2026

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
07:38

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Published on: September 25, 2017

Supramolecular sensor for cancer-associated nitrosamines.

Tsuyoshi Minami1, Nina A Esipenko, Ben Zhang

  • 1Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, United States.

Journal of the American Chemical Society
|December 1, 2012
PubMed
Summary

This study introduces a new fluorescent assay for detecting and quantifying nitrosamines, including cancer-linked types. The method uses cucurbit[n]uril probes to identify these hard-to-detect compounds with high accuracy.

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Area of Science:

  • Supramolecular Chemistry
  • Analytical Chemistry
  • Chemical Sensing

Background:

  • Nitrosamines are challenging to detect and quantify, particularly those linked to cancer.
  • Existing methods for nitrosamine detection often lack sensitivity or specificity.

Purpose of the Study:

  • To develop a novel supramolecular assay for the recognition and quantification of nitrosamines.
  • To address the difficulty in detecting cancer-associated nitrosamines.

Main Methods:

  • Utilized a supramolecular assay incorporating two fluorescent cucurbit[n]uril probes.
  • Leveraged weak interactions and competitive binding among probes, metal ions, and guest molecules.
  • Employed a cross-reactive sensor design.

Main Results:

  • Successfully enabled the recognition and quantification of various nitrosamines.
  • Demonstrated high information density in signal output through variance.
  • Achieved recognition of structurally similar guest molecules, indicating high specificity.

Conclusions:

  • The developed supramolecular assay offers a sensitive and specific method for nitrosamine detection.
  • This approach provides a promising tool for identifying cancer-associated nitrosamines.
  • The sensor's design allows for the differentiation of similar chemical structures.