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Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

V A Huss1, H Festl, K H Schleifer

  • 1Lehrstuhl für Mikrobiologie, Technische Universität München, 8000 München 2, Federal Republic of Germany.

Systematic and Applied Microbiology
|December 1, 2012
PubMed

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...