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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Published on: January 11, 2017
Embryo Proteins and Poly(A)(+) RNA in two Physiological Lines of × Haynaldoticum sardoum During Seed Development and
L Lioi1, I Grilli, M C Anguillesi
1Istituto del Germoplasma, C.N.R., Via Amendola 165/A, I-70126 Bari (Italy).
Abstract:
The content of extractable proteins and of poly(A)(+) RNA in the embryos of two different physiological lines (CP, CV) of × Haynaldoticum sardoum Meletti et Onnis was evaluated at different stages of seed ripening and in after-ripe seeds. The two lines differ in their pattern of protein accumulation; moreover their ability to synthesize polipeptides in the course of seed development is different. The CP and CV seeds are characterized by a different «elative» dormancy: on this basis certain correlations between germination and growth pattern and protein and poly(A)(+) RNA metabolism are hypothesized.
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