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Updated: May 16, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Published on: January 11, 2019
[Changes of Peroxidase, Catalase, and Superoxide Dismutase Activities in Ozone-fumigated Spinach Leaves]
M Decleire1, W De Cat, L De Temmerman
1Institut de Recherches Chimiques, Tervuren, Belgique.
Abstract:
A sensitive spinach variety, cultivated on nutrient solution, was exposed to lethal and sublethal ozone concentrations for 4 hours. Peroxidase, catalase, and superoxide dismutase (SOD) activities were measured immediately and after one, two, four, and seven days. A significant peroxidase stimulation was observed for seven days at 250 and 125 p.p.b. ozone. Catalase activity increased with the lethal concentration and decreased with the sublethal ones. A superoxide dismutase stimulation still occured after two, four, and seven days, at 60-70, 125, and 250 p.p.b. respectively. In our present experiments, an SOD activity higher than 30 U/g leaves may be considered as a indicator of atmospheric pollution.
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