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Heat shock proteins in Amoeba: I. Effect of high temperature on Amoeba proteus and Amoeba borokensis
L V Kalinina1, I A Khrebtukova, O L Podgornaya
1Institute of Cytology, Academy of Sciences of the USSR, Leningrad, USSR.
Abstract:
A sudden increase of temperature of surrounding medium, even in the sublethal range, induced in Amoeba proteus (strain War) and Amoeba borockensis the synthesis of a new class of proteins, which might be classified as the heat shock proteins (hsp). The new pattern of protein synthesis, determined by incorporation of (35)S-methionine, was detectable within 30 min after beginning of the exposure of the cells to the elevated temperature. Amoeba proteus (strain War) and Amoeba borokensis differ distinctly in thermosensitivity, however, no distinct difference in the rate of the heat shock proteins' synthesis between the studied species was found.
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