Related Experiment Video
Updated: May 16, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
Plant Regeneration with Callus and Protoplasts of Solanum uporo Dun
1Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, S7N 0W9 Canada.
Abstract:
Vigorous plants exceding 1 m in height and producing fruits with seeds have been regenerated from callus derived from hypocotyl segments in less than one year, using B5-media containing 0.5 mg · l(-1) 2,4-D, 0.25 mg · l(-1) kinetin, and 0.5 g · l(-1) casein hydrolyzate for callus formation, MS-media with 0.005 mg · l(-1) NAA and 0.5 mg · l(-1) zeatin for shoot formation, and MS-media with 0.005 mg · l(-1) NAA for rooting and plantlet formation. Of cytokinins tested zeatin was the most effective. Protoplasts from leaves of regenerated shoots cultured in vitro divided and formed callus which again and reliably produced shoots and plantlets when placed on MS-media as specified before.
Related Concept Videos
Plant Tissue Culture
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Cell Culture
Whole Body Regeneration
Asexual Reproduction
