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Study of plant protoplast-bacterial spheroplast and cell interactions by flow cytometry

R A Millman1, P F Lurquin

  • 1Program in Genetics and Cell Biology, Washington State University, Pullman, Washington 99164-4350 USA.

Journal of Plant Physiology
|December 1, 2012
PubMed

Keywords:
plant protoplast interactionsbacterial spheroplast bindingflow cytometry applicationscell interaction analysis

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Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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