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Updated: May 16, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Fluorescence spectroscopy of iron-deficient plants
1Department of Plant Physiology, Eötvös Loránd University Budapest, Muzeum krt 4/a, 1088 Hungary.
Abstract:
Iron deficient cucumber (Cucumis sativus L.) plants and such differently supplied with iron were studied by low-temperature fluorescence emission and excitation spectroscopy. Iron deficiency caused a relative decrease and a blue-shift of the 733 nm emission and a decrease or disappearance of the 700 and 707-710 nm excitation bands which are considered to belong to chlorophyll forms of PSI. The process of iron uptake was observed after readdition of iron to previously iron-stressed plants. Iron-deficient plants (with green cotyledon) were treated with the chelator EDTA which treatment resulted in a small increase of the chlorophyll content but the fluorescence spectra of these plants differed from those of plants supplied with iron: they had blue-shifted maxima. The data presented show that fluorescence spectroscopy is a very sensitive and suitable method for studying iron deficiency, observing - indirectly - iron uptake and its utilization in plants.
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