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Published on: May 16, 2014
Development and characterization of 109 polymorphic EST-SSRs derived from the Chinese bayberry (Myrica rubra,
Shao-Yang Zhang1, Xian Li, Chao Feng
1Laboratory of Fruit Quality Biology/State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development, and Quality Improvement, Zhejiang University, Hangzhou 310058, People's Republic of China.
Premise Of The Study:
In Chinese bayberry (Myrica rubra), the available simple sequence repeat (SSR) markers are insufficient to meet the developing demand for genetic and molecular breeding research. This study was aimed at developing a large number of polymorphic expressed sequence tag (EST)-SSRs from the transcriptome of Chinese bayberry. •
Methods And Results:
Five hundred ninety-four compound EST-SSRs and 5557 noncompound ones were identified from 41239 unigene sequences generated from the transcriptome of M. rubra cv. Biqi. Using 10 Chinese bayberry cultivars, 109 polymorphic EST-SSRs were screened from 412 selected. In total, they generated 389 alleles, with a polymorphism ratio of 93.8%. In addition, it was observed that the polymorphism levels of compound EST-SSRs were somewhat lower than those of noncompound ones. •
Conclusions:
The 109 polymorphic EST-SSRs developed from the Chinese bayberry transcriptome should greatly promote the development of genetic and molecular breeding studies in this as well as other Myricaceae species.
