Jove
Visualize
Contact Us

Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Mechanism of Conjugation01:19

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
Conjugation01:19

Conjugation

Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory mechanism of cysteine-dependent methionine biosynthesis in <i>Bifidobacterium longum</i>: insights into sulfur metabolism in gut microbiota.

Gut microbes·2024
Same author

Viability and Diversity of the Microbial Cultures Available in Retail Kombucha Beverages in the USA.

Foods (Basel, Switzerland)·2024
Same author

Development of an endolysin enzyme and its cell wall-binding domain protein and their applications for biocontrol and rapid detection of Clostridium perfringens in food.

Food chemistry·2020
Same author

Use of Lactococcus lactis as a production system for peptides and enzymes encoded by a Lantibiotic gene cluster from Bifidobacterium longum.

Microbiology (Reading, England)·2018
Same author

<i>Thermus</i> and the Pink Discoloration Defect in Cheese.

mSystems·2016
Same author

Compromised Lactobacillus helveticus starter activity in the presence of facultative heterofermentative Lactobacillus casei DPC6987 results in atypical eye formation in Swiss-type cheese.

Journal of dairy science·2016
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

Developing an efficient and reproducible conjugation-based gene transfer system for bifidobacteria.

Wilfredo Dominguez1, Daniel J O'Sullivan1

  • 1Department of Food Science and Nutrition, Cargill Building for Microbial and Plant Genomics, Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, St Paul, MN 55108-1038, USA.

Microbiology (Reading, England)
|December 1, 2012
PubMed
Summary

A new bacterial conjugation protocol effectively transfers genetic material into Bifidobacterium species, overcoming limitations of previous methods like electroporation for probiotic research.

More Related Videos

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
09:55

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

Related Experiment Videos

Last Updated: May 16, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
09:55

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

Area of Science:

  • Microbiology
  • Probiotics
  • Genetic Engineering

Background:

  • Bifidobacteria are crucial probiotics with significant research interest.
  • Existing molecular techniques for Bifidobacteria, such as electroporation, suffer from low efficiency and strain-specific limitations.
  • Efficient genetic manipulation is essential for advancing Bifidobacterium research and applications.

Purpose of the Study:

  • To develop a novel and efficient method for genetic material transfer into Bifidobacterium species.
  • To establish a reliable conjugation protocol applicable to various Bifidobacterium strains, including those resistant to electroporation.
  • To optimize conjugation efficiency by exploring E. coli to Bifidobacterium ratios and plasmid compatibility.

Main Methods:

  • A conjugation protocol was established using the RP4 conjugative machinery in Escherichia coli WM3064(pBB109).
  • A novel transmissible E. coli-Bifidobacterium shuttle vector, pDOJHR-WD2, was constructed and utilized.
  • Conjugation efficiencies were assessed across different Bifidobacterium species and at varying E. coli to Bifidobacterium ratios.
  • The impact of plasmid incompatibility was investigated using Bifidobacterium longum DJO10A and its derivatives.

Main Results:

  • The pDOJHR-WD2 vector was successfully transferred into multiple Bifidobacterium strains across four species.
  • Transfer efficiencies correlated positively with E. coli to Bifidobacterium ratios, reaching nearly 100% at a 10(5):1 ratio.
  • The incoming vector pDOJHR-WD2 coexisted with incompatible resident plasmids at lower copy numbers, detectable by Southern hybridization.
  • Plasmid curing of Bifidobacterium longum DJO10A resulted in a one-log increase in conjugation efficiency due to reduced plasmid incompatibility.

Conclusions:

  • A robust and efficient conjugation-based gene transfer protocol for Bifidobacterium species has been developed.
  • This method overcomes the limitations of electroporation, enabling genetic manipulation of recalcitrant Bifidobacterium strains.
  • The protocol facilitates the introduction of genetic material without size limitations, advancing probiotic research and development.