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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
Published on: February 3, 2023
Essential roles of 11β-HSD1 in regulating brown adipocyte function
Juan Liu1, Xiaocen Kong, Long Wang
1Department of Gerontology, First Hospital Affiliated to Nanjing Medical University, Nanjing 210029, People's Republic of China.
Journal of Molecular Endocrinology
|December 1, 2012
Summary
Inhibiting 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) boosts brown adipose tissue (BAT) function and gene expression. This suggests 11β-HSD1 inhibition is a potential strategy for obesity treatment.
Area of Science:
- Metabolism and Endocrinology
- Adipose Tissue Biology
Background:
- Brown adipose tissue (BAT) is crucial for increasing energy expenditure and combating obesity.
- 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) influences white adipose tissue (WAT) metabolism and function by amplifying local glucocorticoid activity.
Purpose of the Study:
- To investigate the role of 11β-HSD1 in regulating brown adipose tissue (BAT) function.
- To determine if modulating 11β-HSD1 activity impacts BAT-specific gene expression.
Main Methods:
- Utilized primary brown adipocytes from mice and in vivo models.
- Administered BVT.2733, a selective 11β-HSD1 inhibitor, and employed 11β-HSD1-deficient and overexpressing cell models.
- Analyzed the expression of BAT-specific genes (e.g., UCP1, Cidea, Cox7a1, Cox8b) and genes related to brown fat function.
Main Results:
- Inhibition or deficiency of 11β-HSD1 significantly increased BAT-specific gene expression in primary brown adipocytes.
- Overexpression of 11β-HSD1 decreased BAT-specific gene expression, an effect reversed by BVT.2733.
- In vivo studies in high-fat diet-fed mice treated with BVT.2733 showed a similar upregulation of brown fat function-related genes.
Conclusions:
- 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) is a critical regulator of brown fat function and gene expression.
- Targeting 11β-HSD1, potentially through inhibition, represents a promising therapeutic avenue for obesity prevention and treatment.
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