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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 16, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Glycocapture-based proteomics for secretome analysis.

Zon W Lai1, Edouard C Nice, Oliver Schilling

  • 1Institute for Molecular Medicine and Cell Research, University of Freiburg, Stefan-Meier-Strasse 17, Freiburg, Germany.

Proteomics
|December 1, 2012
PubMed
Summary
This summary is machine-generated.

Altered protein glycosylation is key in disease. This review explores glycoprotein enrichment strategies for analyzing cellular secretomes and cell surface proteomes, aiding biomarker discovery and disease diagnosis.

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Last Updated: May 16, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein glycosylation is the most abundant extracellular posttranslational modification.
  • Secreted glycoproteins are crucial for cellular communication and extracellular processes.
  • Altered glycosylation patterns are hallmarks of various pathologies, making glycoproteins valuable biomarkers.

Purpose of the Study:

  • To review glycoprotein enrichment strategies for quantitative analysis of cellular secretomes and cell surface proteomes.
  • To highlight the potential of secreted glycoproteins as biomarkers for early disease diagnosis and surveillance.
  • To discuss the use of glycan moieties as chemical handles for targeted secretome analysis.

Main Methods:

  • Focus on glycoprotein enrichment strategies.
  • Integration with proteomics-based technologies.
  • Quantitative analysis of cell secretomes and cell surface proteomes.

Main Results:

  • Few studies have systemically investigated cellular secretomes due to complexity.
  • Secretomes are rich sources of therapeutic targets and biomarkers.
  • Many existing cancer biomarkers are glycoproteins.

Conclusions:

  • Focused analysis of secreted glycoproteins offers insights for disease diagnosis and surveillance.
  • Glycan structures can serve as chemical handles to simplify secretome analysis.
  • Enrichment strategies are vital for advancing proteomics in secretome and cell surface studies.