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Related Concept Videos

Bioplastics01:27

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

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Enhanced cell-material interactions on medium-pressure plasma-treated polyhydroxybutyrate/polyhydroxyvalerate.

Tinneke Jacobs1, Heidi Declercq, Nathalie De Geyter

  • 1Research Unit Plasma Technology (RUPT), Department of Applied Physics, Faculty of Engineering, Ghent University, Ghent, Belgium. Tinneke.Jacobs@UGent.be

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|December 1, 2012
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Medium-pressure plasma treatment enhances cell adhesion on biodegradable polyhydroxybutyrate/polyhydroxyvalerate (PHB/PHV) films. Air-based treatments offer an economical and effective solution for improving biomedical material surface properties.

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Area of Science:

  • Biomaterials Science
  • Surface Engineering
  • Plasma Physics

Background:

  • Polyhydroxybutyrate/polyhydroxyvalerate (PHB/PHV) is a biodegradable polyester with potential in biomedical applications like scaffolds and sutures.
  • Suboptimal cell adhesion on PHB/PHV necessitates surface modification for enhanced biocompatibility.
  • Improving cell-material interactions is crucial for the efficacy of PHB/PHV in regenerative medicine.

Purpose of the Study:

  • To investigate the efficacy of medium-pressure dielectric barrier discharge (DBD) plasma treatment in enhancing cell adhesion on PHB/PHV films.
  • To analyze the surface property changes induced by plasma treatment in various atmospheres.
  • To determine the optimal and most economical plasma treatment conditions for biomedical applications.

Main Methods:

  • Medium-pressure DBD plasma treatment applied to PHB/PHV films using different gases (e.g., air).
  • Surface characterization using water contact angle measurements to assess hydrophilicity.
  • Surface chemical analysis via X-ray photoelectron spectroscopy (XPS) to determine elemental composition (oxygen, nitrogen).
  • In vitro cell culture studies using human foreskin fibroblasts to evaluate cell adhesion quantitatively and qualitatively.

Main Results:

  • Plasma treatment significantly increased the hydrophilic character of the PHB/PHV surface, confirmed by reduced water contact angles.
  • XPS analysis indicated an increase in surface oxygen and nitrogen content after plasma exposure.
  • Cell culture tests demonstrated markedly improved cell adhesion (both number and morphology) on plasma-treated PHB/PHV.
  • A confluent cell layer was observed on treated samples within 7 days, indicating enhanced biocompatibility.
  • Differences in cell interaction improvements were negligible across different discharge gases, with air being most cost-effective.

Conclusions:

  • Medium-pressure DBD plasma treatment is an effective method for improving the cell-material interaction of PHB/PHV films.
  • Plasma-induced surface modifications enhance fibroblast adhesion and proliferation, making PHB/PHV more suitable for biomedical applications.
  • Plasma treatment in air presents a cost-effective strategy for surface functionalization of PHB/PHV for tissue engineering and regenerative medicine.