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[Emergence of linezolid-resistant Enterococcus faecalis strains from two inpatients in a pediatric ward]

Shin Nihonyanagi1, Yuzuru Adachi, Tomoyo Onuki

  • 1Department of Medical Laboratory, Kitasato University Hospital.

Kansenshogaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
|December 4, 2012
PubMed

Insights

Three linezolid-resistant Enterococcus faecalis strains were identified in two pediatric patients. Genetic analysis revealed a G2576T mutation, suggesting hospital transmission of the resistant bacteria.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Context:

  • Linezolid is a crucial antibiotic for treating resistant Gram-positive infections.
  • The emergence of linezolid resistance in Enterococcus faecalis poses a significant clinical challenge.
  • Hospital-acquired infections require continuous monitoring for antimicrobial resistance patterns.

Purpose:

  • To report the isolation and characterization of linezolid-resistant Enterococcus faecalis strains.
  • To investigate the genetic basis and potential transmission of linezolid resistance in a pediatric hospital setting.
  • To identify the specific mutation responsible for linezolid resistance in the isolated strains.

Summary:

  • Three linezolid-resistant Enterococcus faecalis strains were isolated from two pediatric inpatients at Kitasato University Hospital in 2011.
  • Two strains were isolated from a patient treated with linezolid, while one strain was isolated from a patient with no prior linezolid exposure, suggesting transmission.
  • All resistant strains exhibited a G2576T mutation in the 23S rRNA gene, confirming a common genetic mechanism for resistance.
  • Identical DNA profiles indicated that the resistant strains originated from a single source, likely transmitted between patients within the same hospital room.

Impact:

  • Highlights the potential for rapid development and spread of linezolid resistance in Enterococcus faecalis.
  • Underscores the importance of infection control measures in preventing the transmission of resistant pathogens in healthcare settings.
  • Provides crucial data on the molecular mechanisms and epidemiological spread of linezolid resistance in pediatric populations.

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