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Updated: May 16, 2026
06:10
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
Redox-regulated growth factor survival signaling
John F Woolley1, Aoife Corcoran, Gillian Groeger
1Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College , Cork, Ireland .
Antioxidants & Redox Signaling
|December 4, 2012
Summary
Reactive oxygen species (ROS) are vital for cell signaling, controlling pathways downstream of growth factors. These ROS levels are tightly regulated by enzymes like NADPH oxidases and peroxiredoxins to modulate cellular functions.
Area of Science:
- Cellular Biology
- Biochemistry
- Signal Transduction
Background:
- Reactive oxygen species (ROS), once considered cellular waste, are now recognized as crucial signaling molecules.
- ROS play essential roles in intracellular signaling cascades, particularly downstream of growth factor receptors.
Purpose of the Study:
- To elucidate the complex mechanisms by which ROS control signal transduction pathways.
- To explore the interplay between ROS production and regulation in cellular signaling.
Main Methods:
- Investigated the role of NADPH oxidases (Noxs) in generating ROS upon growth factor stimulation.
- Examined the function of peroxiredoxins in managing hydrogen peroxide (H2O2) levels.
- Analyzed the impact of H2O2 on redox-controlled phosphatases and downstream signaling targets.
Main Results:
- Growth factor stimulation activates Noxs, leading to extracellular superoxide production and subsequent intracellular H2O2 signaling.
- Receptor-associated peroxiredoxins maintain H2O2 homeostasis, preventing cellular damage.
- H2O2 inactivates specific phosphatases, thereby regulating signal propagation and influencing transcription factors.
Conclusions:
- ROS act as critical regulators of signal propagation and attenuation at multiple levels within cellular pathways.
- The intricate redox signaling network, particularly under growth factor stimulation, presents emerging trends in cellular communication.
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