Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the SARS-CoV-2 genome packaging signal in the nsp12-coding region.

Nature communications·2026
Same author

CRISPR in clinical oncology: translational advances from molecular diagnostics to therapeutics.

Nature reviews. Clinical oncology·2026
Same author

Structural basis for chaperone-guided assembly of RNA-induced silencing complex.

Nature·2026
Same author

CEPI Workshop Report: Applying Disease X Vaccine Library and Knowledge Base Approaches to Severe Fever with Thrombocytopenia Syndrome (SFTS).

Vaccines·2026
Same author

Massive barcode-free chemical screenings enable the discovery of bioactive macrocycles with passive membrane permeability.

Nature communications·2026
Same author

De novo design of potent CRISPR-Cas13 inhibitors.

Nature chemical biology·2026

Related Experiment Video

Updated: May 16, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Bringing single-molecule spectroscopy to macromolecular protein complexes.

Chirlmin Joo1, Mohamed Fareh, V Narry Kim

  • 1Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2628CJ, Delft, The Netherlands. c.joo@tudelft.nl

Trends in Biochemical Sciences
|December 4, 2012
PubMed
Summary

Single-molecule fluorescence techniques now reveal protein complex dynamics in cell extracts. This advancement offers new insights into biological functions and clinical applications.

More Related Videos

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates
06:48

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

Published on: January 5, 2024

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry
06:39

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Published on: January 26, 2024

Related Experiment Videos

Last Updated: May 16, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates
06:48

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

Published on: January 5, 2024

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry
06:39

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Published on: January 26, 2024

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biochemistry

Background:

  • Single-molecule fluorescence spectroscopy provides real-time, nanometer-resolution data.
  • The technique has been widely adopted for studying individual protein dynamics and functions over the last 20 years.

Purpose of the Study:

  • To review recent advancements in applying single-molecule fluorescence to investigate macromolecular protein complexes within cell extracts.
  • To discuss challenges, future prospects, and potential applications of these techniques in biological and clinical research.

Main Methods:

  • Review of recent literature on single-molecule fluorescence spectroscopy applied to protein complexes.
  • Focus on techniques enabling analysis within complex biological samples like cell extracts.

Main Results:

  • Significant progress has been made in extending single-molecule fluorescence techniques to study protein complexes.
  • These methods allow for the investigation of physiologically relevant macromolecular assemblies.

Conclusions:

  • Single-molecule fluorescence is increasingly capable of analyzing complex biological systems.
  • Future developments promise expanded applications in biological and clinical research for understanding protein complex behavior.