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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Amperometric glucose biosensor based on electroconductive hydrogels.

Christian N Kotanen¹, Chaker Tlili, Anthony Guiseppi-Elie

¹Center for Bioelectronics, Biosensors and Biochips (C3B), Clemson University Advanced Materials Center, 100 Technology Drive, Anderson, South Carolina, SC 29625, USA.

|December 4, 2012

Summary

A new method fabricates glucose biosensors using electropolymerized pyrrole and glucose oxidase on hydrogel-coated platinum electrodes. This technique offers precise enzyme immobilization for advanced bioelectronic applications.

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Area of Science:

Electrochemistry
Biomaterials Science
Biosensor Technology

Background:

Enzyme amperometric biosensors are crucial for glucose monitoring.
Developing stable and sensitive biotransducers remains a challenge.
Platinum electrodes offer excellent electrochemical properties for biosensing.

Purpose of the Study:

To establish a viable method for fabricating enzyme amperometric glucose biosensors.
To investigate the performance of biosensors created via electropolymerization of pyrrole in the presence of glucose oxidase onto hydrogel-coated platinum electrodes.
To evaluate the impact of fabrication parameters on biosensor sensitivity and stability.

Main Methods:

Platinum micro- and macro-electrodes were activated and modified with APTMS and ACRL-PEG-NHS.
Electrodes were coated with a polyHEMA hydrogel and UV cross-linked.
Pyrrole (Py) was electropolymerized in the presence of glucose oxidase (GOx) to form the biotransducer.

Main Results:

The fabricated biosensors exhibited glucose responsiveness.
Sensitivity was reduced fourfold compared to directly electropolymerized polypyrrole (PPy) films.
Optimal electropolymerization charge density was 1.0 mC/cm(2), yielding an apparent K(M) of 33 mM.
Biosensors showed interference screening of ascorbic acid and increased sensitivity over 17 days of storage.

Conclusions:

Electropolymerization of pyrrole in the presence of glucose oxidase onto hydrogel-coated platinum electrodes is a viable biotransducer fabrication method.
This technique allows precise enzyme immobilization for biosensor, bioelectronics, and bionics applications.
The developed biosensors demonstrate potential for reliable glucose monitoring with improved stability.