Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the C=O stretching, is...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Light Mitigates Bismuth Toxicity While Sustaining Iron Homeostasis in <i>Lepidium sativum</i> Seedlings.

Plants (Basel, Switzerland)·2026
Same author

Non-destructive forensic identification and quantification of cocaine through sealed packaging using FT-NIR spectroscopy and hyperspectral imaging.

Analytica chimica acta·2026
Same author

Type I interferon-activated NK cells control polycythemia vera in vivo.

Blood·2026
Same author

TGF-β1 and TGF-β2 family members differentially modulate tumor initiation and invasiveness of primary liver cancer in a MMP14-dependent manner.

Carcinogenesis·2026
Same author

The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth.

Communications biology·2026
Same author

DoReMiTra: an R/Bioconductor data package for orchestrating the analysis of radiation transcriptomic studies.

Bioinformatics advances·2026

Related Experiment Video

Updated: May 16, 2026

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

Classification of oat and groat kernels using NIR hyperspectral imaging.

Silvia Serranti1, Daniela Cesare, Federico Marini

  • 1Department of Chemical Engineering Materials & Environment Sapienza-Università di Roma Via Eudossiana 18, 00184 Rome, Italy. silvia.serranti@uniroma1.it

Talanta
|December 4, 2012
PubMed
Summary

This study introduces a hyperspectral imaging (HSI) method to classify oat and hull-less groat kernels with nearly 100% accuracy. This non-destructive technique enhances quality control for oat grains.

More Related Videos

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
10:25

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

Published on: June 28, 2016

Related Experiment Videos

Last Updated: May 16, 2026

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
10:25

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

Published on: June 28, 2016

Area of Science:

  • Agricultural Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Oat groats (hull-less kernels) are a key quality indicator in the oat market.
  • Accurate classification of oat types is crucial for quality control and processing.

Purpose of the Study:

  • To develop and validate an innovative procedure for classifying oat and groat kernels.
  • To assess the feasibility of using hyperspectral imaging (HSI) and chemometrics for this classification task.

Main Methods:

  • Acquired hyperspectral images of oat and groat samples in the near-infrared (NIR) range (1006-1650 nm).
  • Utilized Principal Component Analysis (PCA) for exploratory data analysis.
  • Applied Partial Least Squares-Discriminant Analysis (PLS-DA) to build classification models.
  • Selected optimal wavelengths (1132, 1195, 1608 nm) using a bootstrap-VIP procedure for faster processing.

Main Results:

  • Achieved near 100% prediction accuracy in classifying single oat and groat kernels using HSI.
  • Demonstrated that classification models can be effectively built using only three specific wavelengths.
  • The developed method is objective and non-destructive.

Conclusions:

  • Hyperspectral imaging coupled with chemometrics provides a highly accurate method for oat and groat kernel classification.
  • The identification of key wavelengths enables rapid and efficient classification for industrial applications.
  • This HSI-based approach offers significant potential for quality control and innovative sorting solutions in the oat industry.