Related Experiment Video
Updated: May 16, 2026

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
Inhibition of Penicillium expansum by an oxidative treatment
Luciana Cerioni1, María de Los Ángeles Lazarte, Josefina María Villegas
1Instituto de Química Biológica, Química y Farmacia and Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, UNT, Chacabuco 461, CP T4000ILI, Tucumán, Argentina.
Abstract:
Several oxidizing compounds such as sodium hypochlorite (NaClO) and hydrogen peroxide (H(2)O(2)) are used to control postharvest decay in fresh fruit due to their antimicrobial effects. Here, we applied these compounds in vitro, in the presence of CuSO(4), against Penicillium expansum, causal agent of apple blue mold. MICs were 50 mg L(-1) and 400 mmol L(-1) for NaClO and H(2)O(2), respectively, when these compounds were individually applied to conidia suspensions during 2 min. A combined oxidative treatment (OT) consisting on an incubation with 1 mg L(-1) NaClO and 200 mmol L(-1) H(2)O(2), in the presence of 6 mmol L(-1) CuSO(4), inhibited growth, conidial germination and fungal infectivity on apple. The fractional inhibitory concentration index for the interaction between NaClO and H(2)O(2) in the OT was 0.52 indicating a synergistic effect of the oxidizing compounds. These results suggest that the OT could be an interesting alternative for apple diseases postharvest control.
More Related Videos
Related Concept Videos
Production of Antibiotics
Radical Autoxidation
Inhibitors of Gram-positive Cell Wall Synthesis
Antimicrobial Effectiveness
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

