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Updated: May 16, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Full-length single-stranded PCR product mediated chromosomal integration in intact Bacillus subtilis
Sai Wen1, Jianguo Yang, Tianwei Tan
1Beijing Key Laboratory of Bioprocess, The Biorefinery Research and Engineering Center of the Ministry of Education of China, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, PR China.
Abstract:
The research introduced a novel method for gene replacement in intact Bacillus subtilis by employing full-length single-stranded (ss) DNA constructs and electro-transformation. 5' phosphorothioated lagging-strand targeting ssDNA construct was demonstrated to be highly recombinogenic, and the utility of the system was illustrated by introducing a heterologous lipase YlLip2 into amyE locus of B. subtilis through our method.

