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Sandwich-structured upconversion nanoparticles with tunable color for multiplexed cell labeling.

Qingqing Dou¹, Niagara Muhammad Idris, Yong Zhang

¹Department of Bioengineering, National University of Singapore, Singapore 117574, Singapore.

|December 4, 2012

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Researchers developed novel sandwich-structured upconversion nanoparticles (UCNs) for enhanced fluorescence. These multicolor UCNs enable sensitive multiplex detection of subcellular targets using a single near-infrared excitation wavelength.

Area of Science:

Materials Science
Nanotechnology
Biotechnology

Background:

Upconversion nanoparticles (UCNs) offer potential as biological labels due to their unique optical properties.
Existing UCNs often suffer from weak fluorescence or limited color palettes, hindering their application in multiplexing.
There is a need for improved UCNs with enhanced efficiency and tunable colors for advanced biological imaging.

Purpose of the Study:

To design and synthesize novel sandwich-structured UCNs with improved fluorescence efficiency.
To achieve tunable multicolor emissions from UCNs for versatile applications.
To demonstrate the utility of these UCNs in multiplexed detection of subcellular targets.

Main Methods:

Fabrication of sandwich-structured UCNs using a NaYbF(4) core and NaYF(4) shells.

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Doping different emitter ions into the shells to achieve multicolor emission.

Tuning shell thickness and emitter concentration to control color output based on the RGB model.

Utilizing a single near-infrared excitation wavelength for multicolor emission.

Main Results:

Successfully synthesized sandwich-structured UCNs with significantly enhanced fluorescence intensity.
Achieved tunable multicolor emissions covering the RGB spectrum by adjusting doping and shell thickness.
Demonstrated efficient multiplex detection of three subcellular targets using the developed multicolor UCNs.
Confirmed efficient energy transfer from the NaYbF(4) absorber layer to the NaYF(4) emitting layers.

Conclusions:

Sandwich-structured UCNs represent a promising platform for developing highly efficient and multicolor biological labels.
The facile synthesis and tunable emission properties of these UCNs are suitable for advanced multiplexing applications.
These UCNs offer a powerful tool for sensitive and specific multiplex detection in biological systems.