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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

Updated: May 16, 2026

Scattering And Absorption of Light in Planetary Regoliths
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Published on: July 1, 2019

Deep and optically resolved imaging through scattering media by space-reversed propagation.

W Glastre1, O Jacquin, O Hugon

  • 1Centre National de la Recherche Scientifique/Université de Grenoble 1, Laboratoire Interdisciplinaire de Physique, UMR 5588, Grenoble F-3804, France. wglastre@ujf‑grenoble.fr

Optics Letters
|December 4, 2012
PubMed
Summary

This study introduces a new microscopy technique combining laser optical feedback imaging with acoustic photon tagging and synthetic aperture refocusing. It achieves high-resolution imaging deep within scattering tissues, overcoming objective working distance limitations.

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Area of Science:

  • Biomedical Optics
  • Microscopy Techniques
  • Photonics

Background:

  • Scattering and limited working distance impede deep tissue imaging in conventional microscopy.
  • Preserving tissue viability requires low laser power, posing challenges for image quality.

Purpose of the Study:

  • To develop a novel microscopy technique overcoming scattering and objective working distance limitations.
  • To achieve high resolution and sensitivity at low laser power for deep tissue imaging.

Main Methods:

  • Integration of laser optical feedback imaging with acoustic photon tagging.
  • Application of synthetic aperture refocusing for image reconstruction.
  • Demonstration of imaging beyond the microscope's working distance.

Main Results:

  • Achieved ultimate shot-noise sensitivity at low laser power (10 mW).
  • Obtained micrometer resolution over approximately eight transport mean free paths.
  • Extended imaging capability to 1.3 times the microscope's working distance.

Conclusions:

  • The novel microscopy technique effectively penetrates scattering media.
  • This method enables high-resolution, deep-tissue imaging crucial for biomedical applications.
  • Potential applications include advanced biomedical diagnosis and drug development.