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Updated: May 16, 2026
08:47
Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
Published on: March 6, 2019
Thiol reactive probes and chemosensors
Hanjing Peng1, Weixuan Chen, Yunfeng Cheng
1Department of Chemistry, Center for Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30302, USA. hpeng2@gsu.edu
Sensors (Basel, Switzerland)
|December 4, 2012
Summary
This review details fluorescent and colorimetric probes for detecting vital thiol molecules like cysteine and hydrogen sulfide. These sensors are crucial for environmental monitoring and diagnosing diseases through selective thiol analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Environmental Science
Background:
- Thiols, including cysteine (Cys), homocysteine (Hcy), glutathione (GSH), and hydrogen sulfide (H2S), are biologically and environmentally significant.
- These molecules play critical roles in numerous physiological and pathological processes.
- Selective detection of thiols is essential for basic research and clinical diagnostics.
Purpose of the Study:
- To review the design strategies of fluorescent and colorimetric probes and sensors for thiol detection.
- To discuss the mechanisms of action for various thiol detection methods.
- To highlight the importance of selective thiol detection in biological and environmental contexts.
Main Methods:
- Focus on reaction-based probes and sensors utilizing nucleophilic addition/substitution, Michael addition, and bond cleavage (disulfide, Se-N).
- Exploration of metal-sulfur interactions for thiol sensing.
- Specific strategies for hydrogen sulfide (H2S) detection, including nucleophilic cyclization, reduction, and metal sulfide formation.
Main Results:
- Comprehensive overview of diverse probe and sensor designs for thiol detection.
- Detailed discussion on the mechanisms underlying different detection strategies.
- Emphasis on the versatility of probes for detecting various thiols, including H2S.
Conclusions:
- Fluorescent and colorimetric probes offer powerful tools for selective thiol detection.
- Understanding probe design and mechanisms is key to advancing thiol analysis in research and diagnostics.
- Development of novel sensors will continue to impact environmental monitoring and disease diagnosis.
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