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Sensitive electrochemical thrombin aptasensor based on gold disk microelectrodearrays.

Huei-Yu Bai1, F Javier Del Campo, Yu-Chen Tsai

  • 1Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.

Biosensors & Bioelectronics
|December 4, 2012
PubMed
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A new electrochemical aptasensor was developed for sensitive thrombin detection. This biosensor utilizes gold microelectrodes and methylene blue to achieve a low detection limit for thrombin, enabling precise medical diagnostics.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Biotechnology

Background:

  • Electrochemical aptasensors offer advantages for biomolecule detection, including small sample volumes and enhanced mass transport.
  • Gold microelectrode arrays are suitable transducers for aptasensor development due to their favorable electrochemical properties.
  • Thrombin detection is crucial for diagnosing and monitoring various hemostatic and thrombotic disorders.

Purpose of the Study:

  • To construct a sensitive electrochemical aptasensor for the detection of thrombin.
  • To investigate the use of gold disk microelectrode arrays as a transducer platform for aptasensors.
  • To establish a quantitative method for thrombin concentration determination using electrochemical signals.

Main Methods:

  • Fabrication and characterization of gold disk microelectrode arrays using scanning electron microscopy.

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  • Development of an aptasensor based on the interaction between a thrombin aptamer and thrombin.
  • Electrochemical detection using differential pulse voltammetry with methylene blue as an indicator.

    • Main Results:

    • The aptasensor demonstrated a significant decrease in methylene blue peak current upon thrombin binding, indicating displacement.
    • A wide dynamic range for thrombin detection was achieved, spanning from 10⁻⁵ M to 10⁻¹² M.
    • A highly sensitive detection limit of 0.143 pM for thrombin was obtained, showcasing the method's efficacy.

    Conclusions:

    • The developed electrochemical aptasensor provides a sensitive, selective, and reliable method for thrombin detection.
    • Gold microelectrode arrays are effective transducers for aptasensor applications, enabling precise quantitative analysis.
    • This aptasensor holds potential for clinical diagnostics and research involving thrombin level monitoring.