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Updated: May 16, 2026

Preparation of Naringenin Solution for In Vivo Application
Published on: August 10, 2021
Effect of β-cyclodextrin complexation on solubility and enzymatic conversion of naringin
Li Cui1, Zhen-Hai Zhang, E Sun
1Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, Jiangsu, China. xiaobinjia_nj@126.com.
Abstract:
In the present paper, the effect of beta-cyclodextrin (beta-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/beta-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL(-1), and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that beta-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin.
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