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Related Experiment Videos

Xanthine oxidase during human fetal development.

K Vettenranta1, K O Raivio

  • 1Children's Hospital, University of Helsinki, Finland.

Pediatric Research
|March 1, 1990
PubMed
Summary
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Xanthine oxidase (XOD) activity is present in fetal human liver and intestine, increasing in the liver and decreasing in the intestine with gestation. XOD was undetectable in fetal brain and myocardium.

Area of Science:

  • Biochemistry
  • Neonatal Physiology
  • Enzymology

Background:

  • Xanthine oxidase (XOD) contributes to tissue injury via oxygen free-radical production, particularly in newborns.
  • Understanding XOD's role in fetal development is crucial for addressing neonatal pathologies.

Purpose of the Study:

  • To measure and characterize xanthine oxidase (XOD) activity and kinetics in various human fetal tissues.
  • To investigate the developmental changes in XOD activity throughout gestation.

Main Methods:

  • Enzyme activity assays were performed on human fetal liver, intestine, brain, and myocardium.
  • Kinetic parameters, including apparent Km for hypoxanthine, were determined.

Main Results:

  • High XOD activity was detected in fetal liver and intestine throughout gestation.

Related Experiment Videos

  • Liver XOD activity increased with advancing gestation, while intestinal activity decreased.
  • XOD activity was undetectable in fetal brain and myocardium.

    • Conclusions:

    • Fetal liver and intestine possess significant XOD activity, capable of metabolizing hypoxanthine and xanthine.
    • Developmental regulation of XOD activity varies between fetal liver and intestine.
    • The absence of XOD in fetal brain and myocardium suggests limited involvement in these tissues during development.