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Updated: May 16, 2026
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
Published on: January 25, 2017
Autofluorescence as a tool for structural analysis of biofilms formed by nonpigmented rapidly growing mycobacteria
María-Carmen Muñoz-Egea1, María García-Pedrazuela, Ignacio Mahillo
1Departments of Clinical Microbiology, IIS-Fundación Jiménez Díaz.
Abstract:
The structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria.
