Related Experiment Video
Updated: May 16, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
Published on: December 20, 2017
The XA21 binding protein XB25 is required for maintaining XA21-mediated disease resistance
Yingnan Jiang1, Xiuhua Chen, Xiaodong Ding
1Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA.
Abstract:
Plant genomes encode a large number of proteins that potentially function as immune receptors in the defense against pathogen invasion. As a well-characterized receptor kinase consisting of 23 tandem leucine-rich repeats, a transmembrane domain and a serine/threonine kinase, the rice (Oryza sativa) protein XA21 confers resistance to a broad spectrum of Xanthomonas oryzae pv. oryzae (Xoo) races that cause bacterial blight disease. We report here that XA21 binding protein 25 (XB25) belongs to the plant-specific ankyrin-repeat (PANK) family. XB25 physically interacts, in vitro, with the transmembrane domain of XA21 through its N-terminal binding to transmembrane and positively charged domain (BTMP) repeats. In addition, XB25 associates with XA21 in planta. The downregulation of Xb25 results in reduced levels of XA21 and compromised XA21-mediated disease resistance at the adult stage. Moreover, the accumulation of XB25 is induced by Xoo infection. Taken together, these results indicate that XB25 is required for maintaining XA21-mediated disease resistance.
Related Concept Videos
Inheritance of Chromatin Structures
X-Inactivation
X-inactivation
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Dosage Compensation
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
X-linked Traits

