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Related Experiment Video

Updated: May 16, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Dental caries from a molecular microbiological perspective.

B Nyvad¹, W Crielaard, A Mira

¹Department of Dentistry, HEALTH, Aarhus University, Aarhus, Denmark. nyvad@odont.au.dk

Caries Research

|December 5, 2012

Summary

Understanding dental caries requires analyzing the dental biofilm. High-throughput sequencing and metabolome analysis offer new insights into biofilm composition and metabolic activity for better caries research.

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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

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Area of Science:

Microbiology
Genomics
Biochemistry

Background:

Dental caries arise from metabolic imbalances within the dental biofilm.
Traditional culture-based methods and 16S rRNA gene sequencing have limitations in fully characterizing oral biofilms.
Next-generation sequencing offers advanced approaches for studying biofilm composition and function.

Purpose of the Study:

To review and discuss high-throughput sequencing methods for studying dental caries biofilms.
To highlight the importance of metabolic activity and metabolome analysis in understanding caries.
To emphasize the need for precise, site-specific biofilm sampling.

Main Methods:

Review of current high-throughput sequencing technologies (metagenomics, metatranscriptomics).
Discussion of metabolome analysis as a tool for assessing biofilm metabolic activity.
Emphasis on site-specific sampling techniques for biofilm analysis.

Main Results:

High-throughput sequencing provides a deeper understanding of biofilm composition and function.
Metabolome analysis can reveal the metabolic output of the biofilm.
Precise sampling is crucial due to the localized nature of caries.

Conclusions:

An integrated approach combining culture-based studies with advanced molecular techniques is essential.
Understanding the biological function and metabolic output of the dental biofilm is key to fully understanding dental caries.
Future research should utilize comprehensive methods for accurate biofilm analysis in caries.