Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Where Your Eyes Go: How AI Output Design Impacts Reading Behavior.

Journal of imaging informatics in medicine·2026

Same author

Deep Active Learning for Lung Disease Severity Classification from Chest X-rays: Learning with Less Data in the Presence of Class Imbalance.

Journal of imaging informatics in medicine·2025

Same author

HiLAB: A Hybrid Inverse-Design Framework.

Small methods·2025

Same author

Photonic crystal microring resonators on a hybrid silicon nitride-on-lithium niobate platform.

Optics letters·2025

Same author

Synergistic Cancer Therapies Enhanced by Nanoparticles: Advancing Nanomedicine Through Multimodal Strategies.

Pharmaceutics·2025

Same author

Ion-Assisted Nanoscale Material Engineering in Atomic Layers.

Nano letters·2025

Search research articles

Related Experiment Video

Updated: May 16, 2026

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Published on: June 2, 2009

Total variation regularization for 3D reconstruction in fluorescence tomography: experimental phantom studies.

Ali Behrooz¹, Hao-Min Zhou, Ali A Eftekhar

¹School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Dr., Atlanta, Georgia 30332, USA.

|December 5, 2012

Summary

This study introduces total variation (TV) regularization for fluorescence tomography (FT) to improve 3D reconstructions. The new method enhances resolution and accurately localizes fluorescence in biological tissues.

More Related Videos

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Related Experiment Videos

Last Updated: May 16, 2026

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Published on: June 2, 2009

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Area of Science:

Biomedical Optics
Medical Imaging
Computational Biology

Background:

Fluorescence tomography (FT) reconstructs 3D fluorescence distribution in tissues but is ill-posed.
Conventional L2 regularization oversmooths reconstructions, losing critical high-frequency details.
Poor resolution limits the accuracy of depth-resolved fluorescence localization and quantification.

Purpose of the Study:

To develop and evaluate a novel regularization method for FT that preserves sharp features.
To improve the resolution and accuracy of 3D fluorescence reconstructions in biological tissues.
To overcome the ill-posed nature of FT using total variation (TV) regularization.

Main Methods:

Proposed an alternative regularization scheme for FT using the total variation (TV) norm.
Developed two iterative algorithms for fast 3D reconstruction based on TV regularization.
Validated the method using a phantom experiment with a noncontact trans-illumination FT system.

Main Results:

The TV regularization method successfully preserved sharp transitions in fluorescence distribution.
The proposed iterative algorithms enabled fast and accurate 3D FT reconstructions.
Phantom experiments demonstrated superior performance in resolving fluorescence inclusions at various depths compared to conventional methods.

Conclusions:

Total variation (TV) regularization offers a significant improvement over L2 methods for FT.
The developed iterative algorithms provide efficient and high-resolution 3D fluorescence reconstructions.
This approach enhances the capability of FT for accurate biological tissue analysis.