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Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
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Related Experiment Video

Updated: May 16, 2026

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

EGFR gene mutation study in cytology specimens.

Edmond S K Ma1, Wai-Kuen Ng, Chris L P Wong

  • 1Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, China.

Acta Cytologica
|December 5, 2012
PubMed
Summary

Testing for epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer patients using cytology specimens is feasible. This approach supports personalized oncology care for advanced lung cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pathology

Background:

  • Activating mutations in the epidermal growth factor receptor (EGFR) gene predict response to EGFR tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC).
  • Testing for EGFR mutations is recommended for patients with advanced NSCLC considered for first-line TKI therapy.
  • Cytology specimens are crucial for molecular testing in patients with advanced NSCLC unsuitable for surgery.

Purpose of the Study:

  • To evaluate the feasibility and utility of EGFR mutation testing using cytology specimens in a clinical setting.
  • To assess the adequacy and performance of EGFR mutation tests on cytology versus surgical specimens.
  • To highlight the role of cytopathology in personalized oncology.

Main Methods:

  • Retrospective review of 1,410 EGFR mutation testing requests.

Related Experiment Videos

Last Updated: May 16, 2026

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

  • Analysis of 269 cytology specimens (bronchoscopy, body fluid drainage, fine-needle aspiration) and 1,141 surgical specimens.
  • Evaluation of material inadequacy and potential solutions for cytology specimens.

    • Main Results:

    • Cytology specimens can be effectively used for EGFR mutation testing in NSCLC.
    • Challenges with material inadequacy in cytology specimens can be addressed through enrichment strategies and sensitive detection methods.
    • EGFR mutation testing on cytology samples supports timely treatment decisions for advanced NSCLC patients.

    Conclusions:

    • Cytology specimens are a valuable resource for EGFR mutation testing in NSCLC, particularly for advanced disease.
    • Advancements in techniques can overcome limitations of cytology specimens for molecular biomarker testing.
    • Close collaboration between cytopathologists and clinicians is essential for effective personalized cancer care.