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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Triterpene saponins from Clethra barbinervis and their hyaluronidase inhibitory activities
Toshihiro Murata1, Atsushi Suzuki, Nagisa Mafune
1Department of Pharmacognosy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Sendai 981-8558, Japan. murata-t@tohoku-pharm.ac.jp
Abstract:
An extract of Clethra barbinervis with an inhibitory effect on hyaluronidase activity was fractionated guided by the results of an assay. From the active fractions, seven new triterpene saponins (1-4, 6-8) and a new lignan glycoside (14) were isolated together with 14 known compounds (5, 9-13, 15-22). Some of the saponins (2, 3, 9) were revealed as hyaluronidase inhibitors similar to epicatechin (17).
