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Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
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Synchronizing protein transport in the secretory pathway

Gaelle Boncompain1,2, Franck Perez1,2

  • 1Research Center, Institut Curie, Paris, France.

Current Protocols in Cell Biology
|December 5, 2012
PubMed
Summary

Researchers developed the retention using selective hooks (RUSH) system to track secretory protein transport. This method enables simultaneous release from the endoplasmic reticulum and monitoring of protein trafficking to the Golgi apparatus and beyond.

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