Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Images: Skin manifestations and radiographic findings of anti-SAE1 positive dermatomyositis.

ACR open rheumatology·2026
Same author

Syndecan-1 Promotes Alveolar Type 2 Epithelial Cell Senescence during Lung Fibrosis.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Anaphase onset requires CKS-1-mediated destruction of securin in meiosis I and cyclin B1 in meiosis II.

The Journal of cell biology·2026
Same author

Single-Cell Analysis Reveals Peripheral Helper T Cells in Rheumatoid Arthritis-Related Interstitial Lung Disease.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Anaphase onset requires CKS-1-mediated destruction of securin in meiosis I and cyclin B1 in meiosis II.

The Journal of cell biology·2026
Same author

T<sub>H</sub>17 cells converted into exT<sub>H</sub>17 cells sustain rheumatoid-like IL-17-independent inflammatory arthritis.

Science immunology·2025

Related Experiment Video

Updated: May 16, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

An evolutionarily conserved innate immunity protein interaction network.

Lesly De Arras1, Amara Seng, Brad Lackford

  • 1Integrated Department of Immunology, National Jewish Health and University of Colorado, Denver, Colorado 80206, USA.

The Journal of Biological Chemistry
|December 5, 2012
PubMed
Summary
This summary is machine-generated.

Researchers identified novel regulators of innate immunity by comparing genomic screens in C. elegans and mouse macrophages. They discovered a protein interaction network crucial for regulating the lipopolysaccharide (LPS) response, impacting various diseases.

More Related Videos

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

Related Experiment Videos

Last Updated: May 16, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

Area of Science:

  • Immunology
  • Genomics
  • Molecular Biology

Background:

  • The innate immune response is vital for fighting infections but also implicated in diseases like sepsis, cancer, and atherosclerosis.
  • Identifying novel regulators of innate immunity is crucial for understanding and treating these conditions.

Purpose of the Study:

  • To discover new regulators of innate immunity.
  • To elucidate the protein interaction network involved in the lipopolysaccharide (LPS) response.

Main Methods:

  • Comparative genomics RNA interference (RNAi) screens were conducted in Caenorhabditis elegans and mouse macrophages.
  • Physical protein interactions were analyzed to construct an innate immunity protein interaction network.
  • RNAi and overexpression studies were performed in mouse cell lines.
  • In vivo validation was carried out using C. elegans and mouse mutants.

Main Results:

  • Numerous candidate regulators of the LPS response were identified.
  • A conserved protein interaction network for innate immunity was uncovered, including canonical TLR4 pathway proteins and novel interacting partners.
  • Nearly all genes within this network were found to modulate innate immune responses in mouse cell lines.
  • The network's importance in innate immunity regulation was validated in vivo.

Conclusions:

  • A novel, conserved protein interaction network plays a significant role in regulating innate immune responses, particularly to LPS.
  • This network offers potential therapeutic targets for diseases associated with innate immunity dysregulation.