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Related Experiment Videos

Structural analyses of human developmentally regulated Vh3 genes.

P P Chen1

  • 1Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037.

Scandinavian Journal of Immunology
|March 1, 1990
PubMed
Summary

Early B-lymphocyte development involves specific Vh gene expression. Researchers found enhancer-like sequences in human Vh3 genes, suggesting cis-regulatory elements play a role beyond gene proximity.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Early B-lymphocyte development shows preferential expression of specific Vh genes in mice.
  • Human fetal liver Ig cDNA analysis revealed a restricted Vh gene repertoire from Vh1, Vh3, Vh4, and Vh6 families.

Purpose of the Study:

  • To investigate mechanisms regulating Vh gene expression during early B-lymphocyte development.
  • To characterize human germline Vh3 genes within the developmentally restricted Vh repertoire.

Main Methods:

  • Analysis of human Ig cDNA from fetal liver.
  • Characterization of two human germline Vh3 genes.

Main Results:

  • The distance to Jh genes may not solely dictate early Vh gene expression.

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  • Identified numerous enhancer-like sequences within the characterized human Vh3 genes, homologous to known transcriptional enhancers.

    • Conclusions:

    • Positional effects alone do not fully explain programmed Vh gene expression.
    • Cis-regulatory elements are likely crucial for the programmed expression of certain Vh genes during early B-lymphocyte development.