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Related Experiment Video

Updated: May 16, 2026

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

Will the real Bemisia tabaci please stand up?

Wee Tek Tay1, Gregory A Evans, Laura M Boykin

  • 1CSIRO Ecosystem Sciences, Canberra, Australian Capital Territory, Australia.

Plos One
|December 5, 2012
PubMed
Summary

The original 1889 whitefly specimen, Bemisia tabaci, has been identified using mitochondrial DNA sequencing. This confirms the Mediterranean (Q-biotype) as the true B. tabaci, aiding pest management strategies.

Area of Science:

  • Entomology
  • Molecular Genetics
  • Taxonomy

Background:

  • Bemisia tabaci is a globally significant agricultural pest, currently recognized as a cryptic species complex of at least 34 morphologically indistinguishable species.
  • Previous taxonomic revisions have largely involved synonymizing species under the name Bemisia tabaci.
  • Understanding the precise taxonomy of this complex is crucial for effective pest management.

Purpose of the Study:

  • To identify the original 1889 whitefly specimen (Aleyrodes tabaci) using molecular genetic data.
  • To match the historical specimen to a specific member of the Bemisia tabaci cryptic species complex.
  • To establish a foundation for the reclassification of the Bemisia tabaci complex.

Main Methods:

  • Extraction and amplification of a 496 bp fragment from the 3' end of the mitochondrial DNA cytochrome oxidase one (mtCOI) gene.

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Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique
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Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
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Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System

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Last Updated: May 16, 2026

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique
05:24

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Published on: April 1, 2019

Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
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Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System

Published on: November 1, 2017

  • Sequencing of the amplified DNA fragment from a single whitefly from the 1889 collection.
  • Comparison of the obtained sequence with known haplotypes from Mediterranean locations.

    • Main Results:

    • The 123-year-old specimen was unambiguously assigned to a single haplotype found in Greece and Tunisia.
    • The original Aleyrodes tabaci specimen was identified as the Bemisia tabaci Mediterranean species (erroneously called Q-biotype).
    • The Mediterranean species is confirmed as the true Bemisia tabaci.

    Conclusions:

    • Matching historical museum specimens (syntypes) with known species using molecular data is vital for delimiting cryptic species.
    • This study provides the first step towards reclassifying the Bemisia tabaci complex.
    • Accurate classification of the Bemisia tabaci complex is essential for developing improved management strategies for this major pest.