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Updated: May 16, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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Lighting Up RNA-Cleaving DNAzymes for Biosensing.

Kha Tram1, Pushpinder Kanda, Yingfu Li

  • 1Departments of Biochemistry and Biomedical Sciences and Chemistry and Chemical Biology, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1.

Journal of Nucleic Acids
|December 5, 2012
PubMed
Summary

Catalytic DNA molecules, or DNAzymes, can cleave RNA and are increasingly used in biosensing. This review surveys recent advances in developing DNAzymes as fluorescent reporters for enhanced detection capabilities.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • The in vitro selection technique enables the isolation of functional nucleic acids, such as catalytic DNA molecules (DNAzymes), from random-sequence pools.
  • The first DNAzyme, discovered in 1994, exhibits RNA-cleaving activity, leading to the identification of numerous other RNase-like DNAzymes.
  • These DNAzymes have diverse applications in therapeutics, biosensing, and DNA nanotechnology.

Purpose of the Study:

  • To provide a concise survey of significant achievements in developing RNase DNAzymes as fluorescent reporters for biosensing applications.
  • To highlight the progress and potential of DNAzyme-based fluorescent biosensors.

Main Methods:

  • In vitro selection techniques for isolating DNAzymes.
  • Development and characterization of RNase-like DNAzymes.

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Last Updated: May 16, 2026

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  • Integration of DNAzymes into fluorescent reporter systems for biosensing.

    • Main Results:

    • Numerous RNase-like DNAzymes have been isolated using in vitro selection.
    • Significant progress has been made in utilizing these DNAzymes as fluorescent reporters.
    • DNAzyme-based fluorescent biosensors demonstrate promising applications in various research areas.

    Conclusions:

    • RNase DNAzymes are versatile tools with substantial potential in biosensing.
    • The development of DNAzyme-based fluorescent reporters represents a key advancement in biosensor technology.
    • Continued research in this area is expected to yield further innovations in diagnostics and molecular detection.