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Recovery balance: a method for estimating losses in a Bacillus anthracis spore sampling protocol.

S M Da Silva1, A A Urbas, J J Filliben

  • 1Biosystems and Biomaterials Division, Material Measurements Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8315, USA. sdasilva@nist.gov

Journal of Applied Microbiology
|December 6, 2012
PubMed
Summary

A new recovery balance (RB) method quantifies microbial surface sampling performance. This method reveals significant spore loss to wipes, highlighting the need to optimize sampling protocols.

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Surface Science

Background:

  • Microbial surface sampling is crucial for hygiene monitoring.
  • Existing protocols often lack detailed performance assessment.
  • Understanding error sources is key to improving sampling accuracy.

Purpose of the Study:

  • To develop a quantitative method for assessing microbial surface sampling performance.
  • To identify and quantify error contributions within a sampling protocol.
  • To establish a framework for optimizing microbial wipe sampling.

Main Methods:

  • A slip/peel tester ensured consistent pressure during wipe collection.
  • Fluorescence microscopy quantified deposited spores.
  • A recovery balance (RB) method, analogous to mass balance, estimated efficiency at each step.
  • Two studies validated the RB method and assessed parameter impacts.

Main Results:

  • The recovery balance (RB) method successfully quantified performance.
  • Significant spore loss to the wipe material was observed (39.9%).
  • The method demonstrated applicability in evaluating factors like humidity and wipe material.

Conclusions:

  • The recovery balance (RB) method provides a robust approach for analyzing microbial sampling protocols.
  • Substantial spore loss to wipes necessitates further investigation into spore-wipe interactions.
  • This method aids in optimizing laboratory-based sampling protocols and developing new methodologies.