Direct bioanalytical sample injection with 2D LC-MS
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Updated: May 16, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Nm Cassiano1, Jc Barreiro, Rv Oliveira
1Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, 13565-905 São Carlos - SP, Brazil.
Abstract:
New analytical platforms have been developed in response to the need for attaining increased peak capacity for multicomponent complex analysis with higher sensitivity and characterization of the analytes, and high-throughput capabilities. This review outlines the fundamental principles of target and comprehensive 2D LC method development and encompasses applications of LC-LC and LC × LC coupled to MS in bioanalysis using a variety of online analytical procedures. It also provides a rationale for the usage of the most employed mass analyzers and ionization sources on these platforms.
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