Characterization of food spoilage fungi by FTIR spectroscopy
V Shapaval1, J Schmitt, T Møretrø
1Nofima AS, Ås, Norway. volha.shapaval@nofima.no
Aims:
The objective of the study was to evaluate a high-throughput liquid microcultivation protocol and FTIR spectroscopy for the differentiation of food spoilage filamentous fungi.
Methods And Results:
For this study, fifty-nine food-related fungal strains were analysed. The cultivation of fungi was performed in liquid medium in the Bioscreen C microtitre plate system with a throughput of 200 samples per cultivation run. Mycelium was prepared for FTIR analysis by a simple procedure, including a washing and a homogenization step. Hierarchical cluster analysis was used to study affinity among the different species. Based on the hierarchical cluster analysis, a classification and validation scheme was developed by artificial neural network analysis. The classification network was tested by an independent test set. The results show that 93.9 and 94.0% of the spectra were correctly identified at the species and genus level, respectively.
Conclusions:
The use of high-throughput liquid microcultivation protocol combined with FTIR spectroscopy and artificial neural network analysis allows differentiation of food spoilage fungi on the phylum, genus and species level.
Significance And Impact Of The Study:
The high-throughput liquid microcultivation protocol combined with FTIR spectroscopy can be used for the detection, classification and even identification of food-related filamentous fungi. Advantages of the method are high-throughput characteristics, high sensitivity, low costs and relatively short time of analysis.
Related Concept Videos
Microbial Spoilage of Food
IR Frequency Region: Fingerprint Region
The...
Applications of IR Spectroscopy: Overview
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
IR Spectrometers
IR and UV–Vis Spectroscopy of Carboxylic Acids
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...


