Evaluation of diverse antioxidant activities of Galium aparine

Methanol extract and its n-hexane, ethyl acetate, butanol and aqueous fraction of Galium aparine L. (Rubiacea) were evaluated in vitro for their antioxidant capacity (DPPH, superoxide radical, phosphomolybdate assay); reducing power (ABTS, hydroxyl, hydrogen peroxide, to reduce Fe(3+) to Fe(2+) ions) and to estimate total flavonoid and phenolic contents. All the free radical generating assay models depicted differential positive scavenging activity but considerable magnitude for all the fractions. The results showed that aqueous fraction strongly scavenge the DPPH, ABTS, hydroxyl, hydrogen peroxide and superoxide radicals. A significantly high correlation coefficient existed between IC(50) values of DPPH and superoxide radical with total phenolic content and phosphomolybdate assay with total flavonoid contents, respectively. These results suggested that aqueous fraction can be a good source of antioxidant therapeutic in oxidative stress damages.